A phylogenetic tree was generated using neighborjoining analysis with mega software. Pdf the effect of cotton leaf curl burewala virus clcubuv on cotton growth, yield, photosynthetic gas exchange, chlorophyll fluorescence and. Leaf curl, yellow leaf curl hosts tomato, pepper, eggplant, okra, tobacco, beans, cotton, cucurbits, citrus, and other crops as secondary. A cotton leaf curl disease epidemic in the indian subcontinent was first reported in 1967 from multan, pakistan. Cotton leaf curl virus disease clcuvd limits cotton production in many cotton growing countries of the world, including pakistan. Development of cotton leaf curl virus resistant transgenic cotton. Interestingly, we could not detect cotton leaf curl kokhran virusburewala strain clcukovbu. Cotton leaf curl burewala virus, clcubv presently named cotton leaf curl kokhran virusburewala is a constraint for cotton production.
Virus specific transcripts have only been mapped for few. Cotton leaf curl disease clcud is the major plant viral constraint to cotton production on the indian subcontinent. Cotton leaf curl burewala virus clcubuv is a whiteflytransmitted monopartite begomovirus and has recombinant genome composed of sequences derived from clcumuv and cotton leaf curl kokhran virus clcukov that were associated with cotton leaf curl disease clcud during 1990s 2426. In 20002001, a recombinant, cotton leaf curl kokhran virusburewala clcukovbu emerged, causing a second outbreak, apparently driven by the widespread cultivation of the clcumvresistant cotton variety that precipitated the emergence of the resistantbreaking burewala strain mahmood et al. All the available cotton cultivars are susceptible to this virus. First detection of cotton leaf curl burewala virus and cognate cotton leaf curl multan betasatellite and gossypium darwinii symptomless alphasatellite in symptomatic luffa cylindrica in pakistan. Clcubv has been originally reported from burewala territory in pakistan as a variant of cotton leaf curl multan virus mahmood et al. Rebound of cotton leaf curl multan virus and its exclusive detection. Cotton leaf curl virus belongs to begomovirus group, family, geminivirideae. Begomovirus, cotton leaf curl disease, cotton leaf curl burewala virus, betasatellite, cotton leaf curl multan betasatellite, transcription background viruses of the geminiviridae.
In africa, the disease is associated with only one single species of cotton leaf curl. Recently, transgenic tobacco resistant to cotton leaf curl burewala virus has. The c1 promoter expressing the ac1 gene, and v1 promoter expressing the av1 gene are located in opposite orientations in the large intergenic region of the cotton leaf curl burewala virus. Viruses free fulltext functional characterization of. A recent example is the emergence in pakistan of the burewala strain of cotton leaf curl virus clcuv, which overcame the genetic resistance in newly released cotton lines in. The betasatellite associated with clcubuv was also a new strain burewala strain, which was a. Evaluation of gossypium species for resistance to cotton leaf curl. Sequences were analyzed using the lasergene software package. During 2011 in burewala, pakistan, plants in a production field of luffa cylindrica ghia tori were infested with the whitefly bemisia.
Government college university, faculty of life sciences, dept. Partial sequences of 258 and fulllength sequences of 22 virus genomes were determined. Molecular characterization and phylogenetic analysis of a variant of highly infectious cotton leaf curl burewala virus associated with clcud from pakistan. Recombination detection programs and phylogenetic analyses. Cotton leaf curl burewala virusmv14c clcubuvpkfort10 2758. We would like to show you a description here but the site wont allow us. Assessment of genetic diversity of cotton genotypes for. In october 2008 and july 2009, some cotton plants were found to develop upward leaf curl. Functional characterization of a bidirectional plant promoter. Towards the end of the epidemic phase, clcud almost entirely disappeared from. During 2011, characteristic symptoms of leaf curl disease were widespread 40%, and the whitefly bemisia tabaci genn. Across pakistan, clcud is currently associated with a single begomovirus cotton leaf curl burewala virus clcubuv and the cottonspecific betasatellite cotton leaf curl.
The symptomatic induction of cotton leaf curl disease clcud by indexing in lra5166, a clcubuv susceptible genotype of cotton that resists cotton leaf curl multan virus clcumuv. Viruses free fulltext functional characterization of a. Ziaurrehman m, herrmann h w, hameed u, haider m s, brown j k, 20. Cotton leaf curl disease clcud is the major plant viral constraint to cotton production on the indian subcontinent 2. Evidence of cotton leaf curl burewala virus variant and its associate. Cotton leaf curl virus clcuv has emerged as a serious threat to cotton plants by causing leaf curl disease and affecting cotton production by making up to 80% loss in north india and pakistan varma and malathi, 2003, mansoor et al.
Based on threshold free energy and maximum complementarity scores and other parameters, several potential gossypium hirsutumencoded mirnas against clcubv genome were annotated. Cotton family malvaceae, genus gossypium is one of the most important crops in china. In 20002001, a recombinant, cotton leaf curl kokhran virusburewala clcukovbu emerged, causing a second outbreak, apparently driven by the widespread cultivation of. Multiple sequence alignments were performed with clustal x software and.
Cotton leaf curl disease clcud, caused by cotton leaf curl burewala virus clcubv, is a major constraint for a successful production of cotton in pakistan. Cotton leaf curl viruses clcuv are a number of plant pathogenic virus species of the family geminiviridae in asia and africa the major disease of cotton is caused by the cotton leaf curl. Cotton leaf curl multan virusderived viral small rnas can. Transcript mapping of cotton leaf curl burewala virus and its.
Further studies suggested that clcubv is a recombinant which consists of sequences derived from cotton leaf curl multan virus and cotton leaf curl kokhran virus. Southwest crop could use better weather agfax 415 coronavirus. Molecular insight into cotton leaf curl geminivirus disease. Diversity of alphasatellites associated with cotton leaf curl disease in. Molecular characterization of cotton infecting begomovirus es and. In the present study, three cotton leaf curl viruses, cotton leaf curl burewala virus clcubuv, cotton leaf curl kokhran virus clcukov and cotton leaf curl multan virus clcumv, and their associated satellites, cotton leaf curl multan betasatellite clcumb and cotton leaf curl multan. Frontiers cotton leaf curl multan virusderived viral small.
Cotton leaf curl disease clcud, a severe constraint on cotton production in the. Cotton leaf curl burewala virus clcubuv is a whiteflytransmitted monopartite begomovirus that infects cotton and has a recombinant genome composed of sequences derived from the cotton leaf curl multan virus clcumuv and cotton leaf curl kokhran virus clcukov 23,24,25. The c1 promoter expressing the ac1 gene, and v1 promoter expressing the av1 gene are located in opposite orientations in the large intergenic region of the cotton leaf curl burewala virus clcubuv genome. Cotton leaf curl burewala virus institute of food and. Association of cotton leaf curl burewala virus and its. Cotton leaf curl viral disease complex caused by cotton leaf curl virus complex begomovirus, geminiviridae. A survey for viruses causing clcud was conducted during the 2009 and 2010 cropping seasons in the northwestern indian cotton growing belt in the states of punjab, haryana and rajasthan. First report of cotton leaf curl virus infecting cotton was from nigeria in 1912. Clcud is primarily caused by begomovirus, cotton leaf curl burewala virus clcubuv, and cotton leaf curl multan betasatellite clcumb. Cotton leaf curl multan virus clcumuv belonging to begomoviruses family geminiviridae can infect cotton and many other agricultural crops. Pdf molecular characterization and phylogenetic analysis. Cotton leaf curl multan virus newly reported to be.
Cotton leaf curl burewala virus clcubuv, belonging to the genus begomovirus, possesses singlestranded monopartite dna genome. Demographic expansion of the predominant bemisia tabaci. Clcud is primarily caused by begomovirus, cotton leaf curl burewala. Prediction of cotton leaf curl virus disease and its management through resistant germplasm and bioproducts. Identification and application of biocontrol agents.
Cotton leaf curl burewala virus clcubuv sushma ponukumati. Cotton leaf curl burewala virus with intact or mutant transcriptional. The bidirectional promoters representing rep and coat protein cp genes of clcubuv were characterized and their efficacy was assayed. A group of whitefly transmitted ssdna viruses with ssdna satellites, causing leaf curl disease of cotton, vegetables, and ornamentals contents july 28, 20 page executive summary and contributors 1 i. Cotton leaf curl multan virus c4 protein suppresses both. Multiple begomoviruses found associated with cotton leaf curl. Surprisingly, clcubuv lacks c2, one of the genes present in all previously characterized begomoviruses. Transcript mapping of cotton leaf curl burewala virus and. Cotton leaf curl viral disease complex caused by cotton. The spread of cotton leaf curl disease in china, india and pakistan is a. Dna satellites associated with cotton leaf curl disease in pakistan. Cotton leaf curl disease clcud is a major limitation to cotton production on the indian subcontinent. Aug 26, 2019 the beginning of the resistance breaking phase can be pinpointed precisely in the early 2000s with the appearance of a very unusual begomovirus strain, clcukov. Wang j, tang y, yang y, ma n, ling x, kan j, he z and.
Molecular characterization and phylogenetic analysis of a variant of highly infectious cotton. Leaf cuticular wax content is involved in cotton leaf curl. Cotton leaf curl disease clcud is caused by a complex comprising of more than eight species in the genus begomovirus family geminiviridae with associated betasatellite and alphasatellites. Diversity, mutation and recombination analysis of cotton leaf curl. Kl42, cotton leaf curl burewala alphasatellite pakistan. Cotton leaf curl disease, caused by singlestranded dna viruses of the genus begomovirus. A group of whitefly transmitted ssdna viruses with ssdna satellites, causing. Wang j, tang y, yang y, ma n, ling x, kan j, he z and zhang b 2016 cotton leaf curl multan virus derived viral small rnas can target cotton genes to promote viral infection. Genome wide identification of cotton gossypium hirsutum. Cotton leaf curl is a destructive disease of cotton and several other malvaceous plant species that is transmitted by b. Cotton leaf curl burewala virus, clcubv presently named cotton leaf curl kokhran virus burewala is a constraint for cotton production.
Cotton leaf curl burewala virus clcubuv is a whiteflytransmitted monopartite begomovirus that infects cotton and has a recombinant genome composed of sequences derived from the cotton leaf curl multan virus clcumuv and cotton leaf curl kokhran virus clcukov 2325. Research article leaf cuticular wax content is involved in cotton leaf curl virus disease resistance in cotton gossypium hirsutum l. A survey for viruses causing clcud was conducted during the 2009 and. Pdf cotton leaf curl virus clcuv disease in pakistan. Cotton leaf curl disease clcud associated with cotton leaf curl virus is a serious threat to cotton production in the indian subcontinent. Dominance of resistancebreaking cotton leaf curl burewala. Towards the end of the epidemic phase, clcud almost entirely disappeared from cotton as resistant cotton varieties, developed by conventional breeding, came into widespread cultivation by farmers.
In the present study, three cotton leaf curl viruses. Cotton leaf curl burewala virus clcubuv is a whiteflytransmitted monopartite begomovirus and has recombinant genome composed of sequences derived from clcumuv and cotton leaf curl. Cotton leaf curl disease clcud outbreaks caused by clcud. First detection of cotton leaf curl burewala virus and. Across pakistan, clcud is currently associated with a single begomovirus cotton leaf curl burewala virus clcubuv and the cotton specific betasatellite cotton leaf curl multan betasatellite clcumub, both of which have recombinant origins. Identification and application of biocontrol agents against. Multiple begomoviruses found associated with cotton leaf. Functional characterization of a strong bidirectional. Following the appearance of the second clcud epidemic in cotton, to present time, a predominant single recombinant begomovirus named as cotton leaf curl kokhran virus burewala clcukovbu. First detection of cotton leaf curl burewala virus and cognate cotton leaf curl multan betasatellite.
Functional characterization of a bidirectional plant. First report of okra enation leaf curl virus and associated. Development of cotton leaf curl virus resistant transgenic. Following the appearance of the second clcud epidemic in cotton, to present time, a predominant single recombinant begomovirus named as cotton leaf curl kokhran virus. The betasatellite associated with clcubuv was also a new strain burewala strain, which was a recombinant of clcumub and tomato leaf curl betasatellite amin et al. Nov 20, 2019 ziaurrehman m, herrmann h w, hameed u, haider m s, brown j k, 20. Research open access transcript mapping of cotton leaf.
Clcud is primarily caused by begomovirus, cotton leaf curl burewala virus clcubuv and cotton leaf curl multan betasatellite clcumb. In october 2008 and july 2009, some cotton plants were found to develop upward leaf curl symptoms accompanied by thickening and often thick dark greening of leaf veins, leafy enations and stunting in a field of the suburb of nanning city, guangxi, china figs. Among these viruses, the burewala strain is the most dominant in india and pakistan. The beginning of the resistance breaking phase can be pinpointed precisely in the early 2000s with the appearance of a very unusual begomovirus strain, clcukov. Pdf physiological response of cotton leaf curl burewala virus. Pdf cotton leaf curl disease clcud, caused by cotton leaf curl burewala virus clcubv, has emerged as a major threat to cotton production in. Pdf molecular characterization and phylogenetic analysis of. Agroinfiltration was used to transiently express putative promoter constructs in nicotiana tabacum and gossypium hirsutum leaves, which was monitored by a gus reporter gene, and revealed. Molecular characterization and phylogenetic analysis of a. Transcript mapping of cotton leaf curl burewala virus and its cognate.
Geminiviral diseases of cotton 127 cotton leaf curl disease a phylogenetic tree based on complete nucleotide sequences of all cotton begomoviruses is shown in figure 3. Cotton leaf curl multan virus newly reported to be associated. Rep and cp promoters of clcubuv and 35s promoter of cauliflower mosaic virus camv were fused with. Efficacy of the predicted mirnas was computationally verified against clcubv genome. Molecular insight into cotton leaf curl geminivirus.
Cotton leaf curl burewala virus clcubuv is a whiteflytransmitted monopartite begomovirus that infects cotton and has a recombinant genome composed of sequences derived from the. In the present study, three cotton leaf curl viruses, cotton leaf curl. Cotton leaf curl virus clcuv has emerged as a serious threat to cotton plants by causing leaf curl disease and affecting cotton production by making up to 80% loss in north india and. In the past, efforts were made to combat this disease by. For example, cotton leaf curl multan virus clcumuv is an important monopartite begomovirus and infects many plant species including cotton and n. Cotton leaf curl disease clcud is a serious disease of cotton on the indian subcontinent. Already the burewala strain is having a severe negative impact on cotton production in india and pakistan. Taxonomy navigation begomovirus all lower taxonomy nodes 7 common name isynonym. Cotton leaf curl viruses clcuv are a number of plant pathogenic virus species of the family geminiviridae in asia and africa the major disease of cotton is caused by the cotton leaf curl geminivirus clcuv. Begomovirus, cotton leaf curl disease, cotton leaf curl burewala virus, betasatellite, cotton leaf curl multan betasatellite, transcription background viruses of the geminiviridae family have circular singlestranded ssdna genomes and classify into four genera according to their host range, insect vector and genome organization 1. Development of cotton leaf curl virus clcuv diagnostic. Cotton leaf curl viral disease complex caused by cotton leaf. Cotton leaf curl burewala virus clcubuv is a whiteflytransmitted monopartite begomovirus that infects cotton and has a recombinant genome composed of sequences. Prediction of cotton leaf curl virus disease and its.
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